Unlocking the Power of RNA Medicines


RNAte directly addresses the need to test new RNA and mRNA products for immunoreactivity in human cell.

The platform places a high priority on human induced pluripotent stem cell (iPSC) models for sensitive and accurate detection of immune responses. The use of iPSC offers a consistent and flexible platform for testing healthy human cells for innate immune reactions in a range of cell types. iPSC models mitigate risk associated with drug candidates and have the potential to accelerate the development pathway for novel RNA and mRNA products.

Leveraging robotic automation in testing services enables high throughput applications for library screening or testing of different conditions. 

Immunomodulatory Compound Screening

  • Screening for innate immune activation across all RNA sensors
  • Detection of innate immune responses to lipids, adjuvants, other diluents, or contaminants
  • Definition of specific signalling pathway activation using knock-out cell lines
  • Human iPSC-derived specific cell types (i.e. Macrophages) for high sensitivity and biological relevance

Screening Specifications

Screening Services

This service menu outlines a two-tiered testing approach, utilising human macrophage cell lines for standard testing and iPSC technology for advanced evaluations, ensuring a thorough examination of immune reactivity, inflammation, and overall product response profile. The service can be directed to the testing of agonistic activities as well as tailored antagonistic effects.

  • Pre-screening for RNA integrity and product purity.
  • Endotoxin contamination screening.
  • Dose response analyses on a panel of pattern recognition receptors (PRRs) in wild-type human macrophage cell line.
Read more

  • Pre-screening for RNA integrity and product purity
  • Endotoxin contamination screening
  • Dose response analyses on extended panel of PRRs in human macrophages derived from iPSC. Other cell types in development
Read more

Assay Complexity and Sensitivity

Clinical Trials Support

Patient samples can be tested for:

  • Pro-inflammatory cytokine release
  • Gene expression by RT-qPCR or next-generation sequencing

Additional assays available

  • Gene expression analyses: RT-qPCR or next-generation sequencing
  • Extended dose responses
  • Determination of RNA sensing pathways engaged using gene specific knockout cell lines
  • Tailored antagonist assays
  • High throughput screening


  • TLR7 gain-of-function genetic variation causes human lupus

    Nature 2022; 605(7909): 349–356.

    DOI: 10.1038/s41586-022-04642-z

  • Rational design of antisense oligonucleotides modulating the activity of TLR7/8 agonists

    Nucleic Acids Res 2020 Jul 27;48(13):7052-7065

    DOI: 10.1093/nar/gkaa523


  • Activation of cytosolic RNA sensors by endogenous ligands: roles in disease pathogenesis

    Front. Immunol. 2023; Vol. 14 Sec. Molecular Innate Immunity


  • The RNA sensor MDA5 detects SARS-CoV-2 infection

    Scientific Reports 2021; 11, 13638


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Discover how we can assist you in achieving optimal outcomes for your studies and understand why we stand out as leaders in our field.

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